1. SECOND CYCLE - MASTER'S DEGREE
  2. THE GRADUATE SCHOOL OF NATURAL AND APPLIED SCIENCES
  3. BIOLOGY DEPARTMENT
  4. 1461 Biology
Course Information Course Learning Outcomes Course's Contribution To Program ECTS Workload Course Details
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COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
BIO 636REAL-TIME PCR IN APPLIED SCIENCE3 + 02nd Semester7,5

COURSE DESCRIPTION
Course Level Master's Degree
Course Type Elective
Course Objective The aim of the course is to provide technical information about Real-Time PCR technology; To share up-to-date information on applications in the fields of Biology, Environment, Food, and Health Sciences. At the end of the term, students who take the course are expected to have all the necessary equipment to practice with Real-Time PCR practically in any field.
Course Content Real-Time PCR history and fluorogenic chemistry (SYBR Green and Probe systems), Standards, controls and standard curve preparation, Determination of target molecule amount, Multiplex PCR. Role of Real Time PCR in routine diagnosis. Measurement of gene expression. Problems and solutions to be encountered while using Real Time PCR.
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES
1Having detailed knowledge about Real-Time PCR methodology
6Having knowledge about the use of Real-Time PCR in the fields of Biology, Environment, Food and Health
3Obtaining detailed information about gene expression studies
4Learning about qualitative detection methods by Real-Time PCR
5Learning about quantitative analysis methods by Real-Time PCR
2Having detailed knowledge about fluorogenic chemistry

COURSE'S CONTRIBUTION TO PROGRAM
PO 01PO 02PO 03PO 04PO 05PO 06PO 07PO 08PO 09PO 10PO 11
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Sub Total           
Contribution00000000000

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)14342
Hours for off-the-classroom study (Pre-study, practice)129108
Mid-terms11919
Final examination12626
Total Work Load

ECTS Credit of the Course





195

7,5
COURSE DETAILS
 Select Year   


 Course TermNoInstructors
Details 2023-2024 Spring1CANER VURAL
Details 2022-2023 Fall1CANER VURAL
Details 2021-2022 Fall1CANER VURAL


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Course Details
Course Code Course Title L+P Hour Course Code Language Of Instruction Course Semester
BIO 636 REAL-TIME PCR IN APPLIED SCIENCE 3 + 0 1 Turkish 2023-2024 Spring
Course Coordinator  E-Mail  Phone Number  Course Location Attendance
Asts. Prof. Dr. CANER VURAL canerv@pau.edu.tr FEN A0118 FEN AB113 %70
Goals The aim of the course is to provide technical information about Real-Time PCR technology; To share up-to-date information on applications in the fields of Biology, Environment, Food, and Health Sciences. At the end of the term, students who take the course are expected to have all the necessary equipment to practice with Real-Time PCR practically in any field.
Content Real-Time PCR history and fluorogenic chemistry (SYBR Green and Probe systems), Standards, controls and standard curve preparation, Determination of target molecule amount, Multiplex PCR. Role of Real Time PCR in routine diagnosis. Measurement of gene expression. Problems and solutions to be encountered while using Real Time PCR.
Topics
WeeksTopics
1 PCR and Real-Time PCR history and working principles
2 Fluorogenic chemistry (non-specific dyes such as SYBR Green etc.)
3 Fluorogenic chemistry (Probe systems)
4 Fluorogenic chemistry (Primer-probe systems)
5 Primer Design
6 Standards, controls and standard curve preparation
7 Determination the amount of target molecule
8 Multiplex PCR
9 The role of Real Time PCR in routine diagnosis
10 Measurement of gene expression
11 Problems and solutions when using Real Time PCR
12 Interactive student presentations
13 Interactive student presentations
14 Interactive student presentations
Materials
Materials are not specified.
Resources
ResourcesResources Language
Real-Time PCR in Microbiology: From Diagnosis to Characterization, Ian M. Mackay, Caister Academic Press, 2007.English
Real-Time PCR, M. Tevfik Dorak, Taylor&Francis , 2007English
Quantitative Real-Time PCR: Methods and Protocols, Roberto Biassoni, Alessandro Raso, Methods in Molecular Biology, Second Edition, 2020Türkçe
Course Assessment
Assesment MethodsPercentage (%)Assesment Methods Title
Final Exam50Final Exam
Midterm Exam50Midterm Exam
L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes