PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
TLA 233	MOLECULAR BIOLOGICAL METHODS	2 + 2	3rd Semester	4

COURSE DESCRIPTION

Course Level	Associate's Degree
Course Type	Compulsory
Course Objective	Gains knowledge about molecular biological methods used in the laboratory and develops basic skills for these methods.
Course Content	Structure of DNA-RNA, isolation and analysis of DNA-RNA, PCR analysis, agarose gel electrophoresis, Southern blot analysis, Northern blot analysis, Western blot analysis, enzyme treatments of DNA, restriction enzymes, RFLP analysis, DNA cloning, DNA sequencing
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	Gain knowledge about molecular biological methods
2	Learns to isolate nucleic acids (DNA-RNA) from various body fluids and tissues and use these molecules in analyses.
3	Have knowledge about molecular biological methods and these methods can be used in the laboratory

COURSE'S CONTRIBUTION TO PROGRAM

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	4	56
Hours for off-the-classroom study (Pre-study, practice)	13	2	26
Mid-terms	1	8	8
Final examination	1	14	14
Total Work Load ECTS Credit of the Course			104 4

COURSE DETAILS

Select Year

	Course Term	No	Instructors
Details	2023-2024 Fall	1	HİLAL BÜŞRA TOKGÖZ BOZKURT
Details	2023-2024 Fall	2	HİLAL BÜŞRA TOKGÖZ BOZKURT

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester
TLA 233	MOLECULAR BIOLOGICAL METHODS	2 + 2	1	Turkish	2023-2024 Fall

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Lecturer HİLAL BÜŞRA TOKGÖZ BOZKURT	hbtokgoz@pau.edu.tr		DSHMY D0010 DSHMY D0312	%70

Goals		Gains knowledge about molecular biological methods used in the laboratory and develops basic skills for these methods.
Content		Structure of DNA-RNA, isolation and analysis of DNA-RNA, PCR analysis, agarose gel electrophoresis, Southern blot analysis, Northern blot analysis, Western blot analysis, enzyme treatments of DNA, restriction enzymes, RFLP analysis, DNA cloning, DNA sequencing

Topics

Weeks	Topics
1	Structure of DNA-RNA
2	İsolation and analyses of DNA-RNA
3	PCR analyses
4	Agarose gel electrophoresis
5	Southern blotting
6	Northern blotting
7	Western blotting
8	Enzymatic manipulation of DNA
9	Recombinant DNA Technologies
10	Recombinant DNA Technologies
11	Restriction enzyms
12	RFLP analyses
13	DNA clonning
14	DNA sequencing methods

Materials

Materials are not specified.

Resources

Resources	Resources Language
	Türkçe

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	60	Final Exam
Midterm Exam	40	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student