1. THIRD CYCLE - DOCTORATE DEGREE
  2. THE GRADUATE SCHOOL OF NATURAL AND APPLIED SCIENCES
  3. BIOLOGY DEPARTMENT
  4. 1741 Biology PhD
Course Information Course Learning Outcomes Course's Contribution To Program ECTS Workload Course Details
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COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
BIO 535NUCLEIC ACID ANALYSES3 + 02nd Semester7,5

COURSE DESCRIPTION
Course Level Doctorate Degree
Course Type Elective
Course Objective All procedures used in molecular biology and biochemistry laboratories for the analysis of nucleic acid are given as much as detailed. The main objective of this course to give fundamental, skills to students to carry out successful experiments.
Course Content Major topics include, Media Preparation and Bacteriological Tools, Introduction to Plasmid Biology, Minipreps & Large-Scale Preparation f, Plasmid DNA, Introduction of Plasmid DNA into Cells, Introduction to Lambda Phages, Preparation of Genomic DNA, Resolution & Recovery of DNA Fragments, Agarose Gel Electrophoresis, Capillary Electrophoresis of DNA, Southern Blotting, Hybridization Analysis of DNA Blots, Restriction Endonucleases & Restriction Mapping, Enzymes for Modifying and Radioactively Labeling Nucleic Acids, Construction of Hybrid DNA Molecules, Preparation & Analysis of RNA from Eukaryotic And Prokaryotic Cells, Production Of Genomic DNA And cDNA Libraries, Specialized Strategies For Screening Libraries, DNA Sequencing.
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES
1Will be able to know how to prepare plasmid
2Will be able to know how to get genomic DNA
3Will be able to know agarose electrophoresis
4Will be able to know southern blotting
5Will be able to know southern blotting
6Will be able to know DNA and RNA sequencing
7Will be able to know mutation analysis

COURSE'S CONTRIBUTION TO PROGRAM
Data not found.

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)14342
Hours for off-the-classroom study (Pre-study, practice)12784
Mid-terms12323
Final examination14646
Total Work Load

ECTS Credit of the Course





195

7,5
COURSE DETAILS
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L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes