PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
BIO 570	EXPERIMENTAL MOLECULAR BIOLOGY	2 + 4	1st Semester	7,5

COURSE DESCRIPTION

Course Level	Doctorate Degree
Course Type	Elective
Course Objective	This course aims to practical application of recombinant DNA methods used in the field of molecular biology and molecular biochemistry in a comprehensive way and accomplish students with these skills.
Course Content	This is a laboratory course that focuses on molecular biology techniques. Research techniques in molecular genetics and nucleic acid biochemistry will be handled: Plasmid isolation, restriction analysis, molecular cloning, PCR, site-directed mutagenesis, overexpression of proteins in E. coli, purification of recombinant proteins, analysis of proteins by SDS-PAGE and western blotting, computer-aided DNA sequence analysis, nucleic acid hybridization. Students will learn not merely how to carry out laboratory procedures, but also how these methods are used to unravel information about genes and proteins.
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	Will be able to do Plasmid isolation.
2	Will be able to perform Restriction mapping.
3	Will be able to do PCR subcloning.
4	Will be able to carry out expression of proteins.
5	Will be able to perform purification of expressed protein.
6	Will be able to carry out 2D Gel electrophoresis.
7	Will be able to do sequence analysis on computer.
8	Will be able to identify specific genes.

COURSE'S CONTRIBUTION TO PROGRAM

Data not found.

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	3	42
Hours for off-the-classroom study (Pre-study, practice)	12	6	72
Mid-terms	1	24	24
Laboratory	4	11	44
Final examination	1	13	13
Total Work Load ECTS Credit of the Course			195 7,5

COURSE DETAILS

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L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student