PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
TBI 622	RNA INTERFERENCE	2 + 0	3rd Semester	5

COURSE DESCRIPTION

Course Level	Doctorate Degree
Course Type	Elective
Course Objective	To learn the significance of RNA interference in regulation of gene expression, the its important role in developmet and to understand the significance within gene's function analysis.
Course Content	RNA interference (RNAi) is a system within living cells that helps to control which genes are active and how active they are. Two types of small RNA molecules – microRNA (miRNA) and small interfering RNA (siRNA) – are central to RNA interference. RNAs are the direct products of genes, and these small RNAs can bind to specific other RNAs and either increase or decrease their activity, for example by preventing a messenger RNA from producing a protein. RNA interference has an important role in defending cells against parasitic genes – viruses and transposons – but also in directing development as well as gene expression in general. At the end of this lecture studends will understand with their hands on experience how can we manuplate the cells with RNA interferance and what is the phenotipical changes of the cells.
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	Describes the functions of small RNAs
2	Explains the difference between post-tanscriptional gene silencing and transcriptional gene silencing
3	Describes different possibilities how to do an RNAi experiment, the advantages and disadvantages of the different options.

COURSE'S CONTRIBUTION TO PROGRAM

	PO 01	PO 02	PO 03	PO 04	PO 05	PO 06	PO 07	PO 08	PO 09	PO 10	PO 11	PO 12
LO 001	2		3	1		3	4	2	1	3	1	2
LO 002	2		3	1		3	4	2	1	3	1	2
LO 003	2	3	3	2	2	4	4	3	1	3	1	3
Sub Total	6	3	9	4	2	10	12	7	3	9	3	7
Contribution	2	1	3	1	1	3	4	2	1	3	1	2

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	2	28
Hours for off-the-classroom study (Pre-study, practice)	14	2	28
Mid-terms	1	30	30
Final examination	1	44	44
Total Work Load ECTS Credit of the Course			130 5

COURSE DETAILS

Select Year

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester
TBI 622	RNA INTERFERENCE	2 + 0	1	Turkish	2022-2023 Fall

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Prof. Dr. YAVUZ DODURGA	ydodurga@pau.edu.tr		KKM A0305-04	%80

Goals		To learn the significance of RNA interference in regulation of gene expression, the its important role in developmet and to understand the significance within gene's function analysis.
Content		RNA interference (RNAi) is a system within living cells that helps to control which genes are active and how active they are. Two types of small RNA molecules – microRNA (miRNA) and small interfering RNA (siRNA) – are central to RNA interference. RNAs are the direct products of genes, and these small RNAs can bind to specific other RNAs and either increase or decrease their activity, for example by preventing a messenger RNA from producing a protein. RNA interference has an important role in defending cells against parasitic genes – viruses and transposons – but also in directing development as well as gene expression in general. At the end of this lecture studends will understand with their hands on experience how can we manuplate the cells with RNA interferance and what is the phenotipical changes of the cells.

Topics

Weeks	Topics
1	The discovery and development of RNA interference
2	RNAi mechanism, micro RNA (miRNA) pathway
3	RNAi in different model organisms principles and applications
4	Comparison of RNAi methodologies
5	siRNA design for mammalian systems
6	Experimental implementation and analysis methods
7	Midterm exam
8	Mammalian cell lines transfected with siRNA
9	siRNA transfection and Data Analysis
10	RNA extraction from transfected cells
11	RT-PCR measurement of the expression of silenced genes by RNAi
12	Design for RNAi experiments
13	siRNA-based medical therapy
14	Final exam

Materials

Materials are not specified.

Resources

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	50	Final Exam
Midterm Exam	50	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student