PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
TBI 613	METHODS IN MOLECULAR BIOLOGY	2 + 2	2nd Semester	5

COURSE DESCRIPTION

Course Level	Doctorate Degree
Course Type	Elective
Course Objective	To understand the theory and practice of fundamental and general laboratory techniques used in molecular biology research, To gain practical skills that are the core of research in molecular biology, To cover the self-reliance in approaching experimental design and record keeping. .
Course Content	Collection and storage of biologic materials. Isolation methods of DNA, RNA, and proteins. Principles of polimerize chain reaction, Protein and DNA electrophoresis, jel staining and imaging methods .
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	Defines the techniques commonly used molecular biology researches.
2	Acquires skills to work independently, successfully and safely in molecular laboratories.
3	Interpret the results of laboratory tests.
4	Acquire the basic skills required to conduct experimental work in the laboratory in the field of Molecular Biology.

COURSE'S CONTRIBUTION TO PROGRAM

	PO 01	PO 02	PO 03	PO 04	PO 05	PO 06	PO 07	PO 08	PO 09	PO 10	PO 11	PO 12
LO 001	1	5	3	3	5	3	3	3	3	3	2	4
LO 002	1	5	3	3	5	3	3	3	3	3	2	4
LO 003	1	5	3	3	5	3	3	3	3	3	2	4
LO 004	1	5	3	3	5	3	3	3	3	3	2	4
Sub Total	4	20	12	12	20	12	12	12	12	12	8	16
Contribution	1	5	3	3	5	3	3	3	3	3	2	4

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	2	28
Hours for off-the-classroom study (Pre-study, practice)	14	2	28
Mid-terms	1	30	30
Final examination	1	44	44
Total Work Load ECTS Credit of the Course			130 5

COURSE DETAILS

Select Year

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester
TBI 613	METHODS IN MOLECULAR BIOLOGY	2 + 2	1	Turkish	2023-2024 Spring

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Prof. Dr. YAVUZ DODURGA	ydodurga@pau.edu.tr		KKM A0314	%80

Goals		To understand the theory and practice of fundamental and general laboratory techniques used in molecular biology research, To gain practical skills that are the core of research in molecular biology, To cover the self-reliance in approaching experimental design and record keeping. .
Content		Collection and storage of biologic materials. Isolation methods of DNA, RNA, and proteins. Principles of polimerize chain reaction, Protein and DNA electrophoresis, jel staining and imaging methods .

Topics

Weeks	Topics
1	General principles of molecular laboratories, work areas and equipments
2	Genomic DNA of eukaryotic cells and bacteria, RNA isolation and plasmid DNA isolation: General strategy
3	Isolation of DNA from different sources (blood, tissue and FFPE tissue samples)
4	Isolation of total RNA from different sources (blood, tissue and FFPE tissue samples) and RT-PCR
5	Polymerase Chain Reaction: Basic principles and applications
6	Restriction endonucleases and using in molecular methods
7	Midterm exam
8	Mutation and polymorphism analysis methods
9	DNA sequence analysis
10	Genome profile
11	Protein production and analysis methods
12	In situ hybridization techniques
13	Article discussion
14	Final exam

Materials

Materials are not specified.

Resources

Resources	Resources Language
Carl W. Dieffenbach (Editor), Gabriela S. Dveksler (Editor) -Pcr Primer: A Laboratory Manual	English
Joe Sambrook, Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set), Cold Spring Harbor Laboratory Press; 3rd edition (January 15, 2001)	English

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	50	Final Exam
Midterm Exam	50	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student