PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
BIO 656	COMPUTATIONAL METHODS FOR MOLECULAR BIOLOGICAL AND BIOTECHNOLOGICAL STUDIES	3 + 0	2nd Semester	7,5

COURSE DESCRIPTION

Course Level	Master's Degree
Course Type	Elective
Course Objective	The aim of the course is to help make basic calculations that may be necessary for Molecular Biological and Biotechnological studies with correct approaches. With the theoretical and practical knowledge obtained at the end of the course, researchers are expected to learn the mathematical and statistical methods necessary to easily solve the basic problems they encounter in scientific studies and thus reach the most accurate result.
Course Content	Scientific Notation and Metric Prefixes, Solutions, Mixtures and Media, Cell Growth: Working with Bacteriophage, Determining the Amount of Nucleic Acids, Labeling Nucleic Acids with Radioisotopes; Oligonucleotide Synthesis: Synthesis Yield; Polymerase Chain Reaction Activity; Measurement of Gene Expression by Real-Time PCR; Calculation of Relative Quantity by Reaction Kinetics in Real-Time PCR; Digital PCR; Recombinant DNA: Quantification of Fragment Ends; Use of β-Galactosidase to Monitor Promoter Activity and Gene Expression; Use of Luciferase in Reporter Analysis; Forensic Medicine and Paternity: Calculating Genotype Frequency.
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	Knowing and applying basic calculations used in molecular biology and biotechnology
2	Being able to interpret the results obtained using the basic analysis methods used in molecular biology and biotechnology
3	Being able to optimize the applications in molecular biology and biotechnology
4	Learning how to put calculations into practice in research and professional fields
5	being able to select, apply and interpret the necessary experimental application for the problems encountered
6	Determination of amount of nucleic acid, determination of oligonucleotide synthesis efficiency and PCR activity in a polymerase chain reaction, determination of relative amount with reaction kinetics in real-time PCR
8	Having detailed computational knowledge about recombinant DNA techniques
9	having knowledge about the use and applications of molecular biological techniques in forensic sciences
7	Having knowledge about digital PCR working principles and application fields

COURSE'S CONTRIBUTION TO PROGRAM

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	3	42
Hours for off-the-classroom study (Pre-study, practice)	12	9	108
Mid-terms	1	19	19
Final examination	1	26	26
Total Work Load ECTS Credit of the Course			195 7,5

COURSE DETAILS

Select Year

	Course Term	No	Instructors
Details	2021-2022 Fall	1	CANER VURAL

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester
BIO 656	COMPUTATIONAL METHODS FOR MOLECULAR BIOLOGICAL AND BIOTECHNOLOGICAL STUDIES	3 + 0	1	Turkish	2021-2022 Fall

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Asts. Prof. Dr. CANER VURAL	canerv@pau.edu.tr		FEN B0213	%80

Goals		The aim of the course is to help make basic calculations that may be necessary for Molecular Biological and Biotechnological studies with correct approaches. With the theoretical and practical knowledge obtained at the end of the course, researchers are expected to learn the mathematical and statistical methods necessary to easily solve the basic problems they encounter in scientific studies and thus reach the most accurate result.
Content		Scientific Notation and Metric Prefixes, Solutions, Mixtures and Media, Cell Growth: Working with Bacteriophage, Determining the Amount of Nucleic Acids, Labeling Nucleic Acids with Radioisotopes; Oligonucleotide Synthesis: Synthesis Yield; Polymerase Chain Reaction Activity; Measurement of Gene Expression by Real-Time PCR; Calculation of Relative Quantity by Reaction Kinetics in Real-Time PCR; Digital PCR; Recombinant DNA: Quantification of Fragment Ends; Use of β-Galactosidase to Monitor Promoter Activity and Gene Expression; Use of Luciferase in Reporter Analysis; Forensic Medicine and Paternity: Calculating Genotype Frequency.

Topics

Weeks	Topics
1	Scientific Notation and Metric Examples
2	Solutions, Mixtures and Media
3	Cell Growth: Working with Bacteriophage
4	Determination of Nucleic Acid Amount
5	Labeling of Nucleic Acids with Radioisotopes
6	Oligonucleotide Synthesis: Synthesis Yield
7	Polymerase Chain Reaction Activity
8	Measuring Gene Expression by Real-Time PCR
9	Relative Quantity Calculation with Reaction Kinetics in Real-Time PCR
10	Digital PCR
11	Recombinant DNA: Calculating the Amount of Fragment Ends
12	Using β-Galactosidase for Monitoring Promotor Activity and Gene Expression
13	Use of Luciferase in Reporter Analysis
14	Forensic Medicine and Paternity: Calculating Genotype Frequency

Materials

Materials are not specified.

Resources

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	50	Final Exam
Midterm Exam	50	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student