PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
BIO 584	MAMMALIAN CELL CULTURE METHODS	3 + 0	1st Semester	7,5

COURSE DESCRIPTION

Course Level	Master's Degree
Course Type	Elective
Course Objective	The purpose of this course is to introduce garduate students to basic mammalian cell culture techniques. The course will provide essential information on the aseptic techniques, and passaging of adherent and non-adherent cells. During the course, the differences between continuous cell lines and primary cells will be discussed. Finally, basic methods for monitoring cell growth and viability will be discussed.
Course Content	Introduction to basic principles, Aseptic techniques, and the use of the vertical laminar-flow biosafety cabinet. Selection and preparation of media and antibiotics, waste disposal. Freezing and thawing of cells, passaging of adherent cells, passaging floater cells, cell counting and determination of cell viability. Cell viability determination. Differences between primary, transformed, and cancer cells. Establishment of primary cultures from tissue. Separation of mononuclear cells from blood. Methods for determining cell cycling, growth and viability. Cell viability assessment by trypan blue exclusion assay. Cell growth determination by MTT assay. Scallng-up of animal cell cultures. Cell line preservation and authentication. Epithelial stem cell Identification, isolation, and culture. Senescence, apoptosis, and necrosis.
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	Will be able to know Aseptic techniques, and the use of the vertical laminar-flow biosafety cabinet
2	Will be able to know the Freezing and thawing of cells, passaging of adherent cells, passaging floater cells, cell counting and determination of cell viability
3	Will be able to know the differences between primary, transformed, and cancer cells.
4	Will be able to do Cell viability assessment by trypan blue exclusion assay.
5	Will be able to do Cell growth determination by MTT assay
6	Will be able to know the Establishment of primary cultures from tissue

COURSE'S CONTRIBUTION TO PROGRAM

	PO 01	PO 02	PO 03	PO 04	PO 05	PO 06	PO 07	PO 08	PO 09	PO 10	PO 11
LO 001	4	5	4	4			5	4	5	4
LO 002	4	5	4	5			3	3	3	4
LO 003	3	4	5			4	3		3	5
LO 004		4	5	3
LO 005	3		4	3
LO 006	3		3	3	4
Sub Total	17	18	25	18	4	4	11	7	11	13
Contribution	3	3	4	3	1	1	2	1	2	2	0

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	3	42
Hours for off-the-classroom study (Pre-study, practice)	14	7	98
Assignments	1	5	5
Mid-terms	1	15	15
Final examination	1	35	35
Total Work Load ECTS Credit of the Course			195 7,5

COURSE DETAILS

Select Year

	Course Term	No	Instructors
Details	2019-2020 Fall	1	ŞEVKİ ARSLAN

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester
BIO 584	MAMMALIAN CELL CULTURE METHODS	3 + 0	1	Turkish	2019-2020 Fall

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Prof. Dr. ŞEVKİ ARSLAN	sevkia@pau.edu.tr		İTBF BB111	%

Goals		The purpose of this course is to introduce garduate students to basic mammalian cell culture techniques. The course will provide essential information on the aseptic techniques, and passaging of adherent and non-adherent cells. During the course, the differences between continuous cell lines and primary cells will be discussed. Finally, basic methods for monitoring cell growth and viability will be discussed.
Content		Introduction to basic principles, Aseptic techniques, and the use of the vertical laminar-flow biosafety cabinet. Selection and preparation of media and antibiotics, waste disposal. Freezing and thawing of cells, passaging of adherent cells, passaging floater cells, cell counting and determination of cell viability. Cell viability determination. Differences between primary, transformed, and cancer cells. Establishment of primary cultures from tissue. Separation of mononuclear cells from blood. Methods for determining cell cycling, growth and viability. Cell viability assessment by trypan blue exclusion assay. Cell growth determination by MTT assay. Scallng-up of animal cell cultures. Cell line preservation and authentication. Epithelial stem cell Identification, isolation, and culture. Senescence, apoptosis, and necrosis.

Topics

Weeks	Topics
1	Introduction to basic principles,
2	Aseptic techniques, and the use of the vertical laminar-flow biosafety cabinet.
3	Selection and preparation of media and antibiotics, waste disposal. Freezing and thawing of cells, passaging of adherent cells, passaging floater cells, cell counting and
4	determination of cell viability. Cell viability determination.
5	Differences between primary, transformed, and cancer cells.
6	Establishment of primary cultures from tissue.
7	Separation of mononuclear cells from blood.
8	Methods for determining cell cycling, growth and viability.
9	Cell viability assessment by trypan blue exclusion assay. Cell growth determination by MTT assay.
10	Scallng-up of animal cell cultures.
11	MIDTERM
12	Cell line preservation and authentication.
13	Epithelial stem cell Identification, isolation, and culture.
14	Senescence, apoptosis, and necrosis.

Materials

Materials are not specified.

Resources

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	50	Final Exam
Midterm Exam	50	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student