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COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
BIO 570EXPERIMENTAL MOLECULAR BIOLOGY2 + 42nd Semester7,5

COURSE DESCRIPTION
Course Level Master's Degree
Course Type Elective
Course Objective This course aims to practical application of recombinant DNA methods used in the field of molecular biology and molecular biochemistry in a comprehensive way and accomplish students with these skills.
Course Content This is a laboratory course that focuses on molecular biology techniques. Research techniques in molecular genetics and nucleic acid biochemistry will be handled: Plasmid isolation, restriction analysis, molecular cloning, PCR, site-directed mutagenesis, overexpression of proteins in E. coli, purification of recombinant proteins, analysis of proteins by SDS-PAGE and western blotting, computer-aided DNA sequence analysis, nucleic acid hybridization. Students will learn not merely how to carry out laboratory procedures, but also how these methods are used to unravel information about genes and proteins.
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES
1Will be able to do Plasmid isolation.
2Will be able to perform Restriction mapping.
3Will be able to do PCR subcloning.
4Will be able to carry out expression of proteins.
5Will be able to perform purification of expressed protein.
6Will be able to carry out 2D Gel electrophoresis.
7Will be able to do sequence analysis on computer.
8Will be able to identify specific genes.

COURSE'S CONTRIBUTION TO PROGRAM
PO 01PO 02PO 03PO 04PO 05PO 06PO 07PO 08PO 09PO 10PO 11
LO 0014454 5454  
LO 0025444 4  44 
LO 003 444       
LO 0044454   344 
LO 005 454       
LO 0065445       
LO 0075  45545   
LO 00855 5       
Sub Total28292734514813128 
Contribution44341212210

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)14342
Hours for off-the-classroom study (Pre-study, practice)14798
Assignments155
Mid-terms11515
Final examination13535
Total Work Load

ECTS Credit of the Course






195

7,5
COURSE DETAILS
 Select Year   


 Course TermNoInstructors
Details 2011-2012 Fall1ALAATTİN ŞEN


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Course Details
Course Code Course Title L+P Hour Course Code Language Of Instruction Course Semester
BIO 570 EXPERIMENTAL MOLECULAR BIOLOGY 2 + 4 1 Turkish 2011-2012 Fall
Course Coordinator  E-Mail  Phone Number  Course Location Attendance
Prof. Dr. ALAATTİN ŞEN sena@pau.edu.tr Course location is not specified. %
Goals This course aims to practical application of recombinant DNA methods used in the field of molecular biology and molecular biochemistry in a comprehensive way and accomplish students with these skills.
Content This is a laboratory course that focuses on molecular biology techniques. Research techniques in molecular genetics and nucleic acid biochemistry will be handled: Plasmid isolation, restriction analysis, molecular cloning, PCR, site-directed mutagenesis, overexpression of proteins in E. coli, purification of recombinant proteins, analysis of proteins by SDS-PAGE and western blotting, computer-aided DNA sequence analysis, nucleic acid hybridization. Students will learn not merely how to carry out laboratory procedures, but also how these methods are used to unravel information about genes and proteins.
Topics
WeeksTopics
1 Introductıon
2 DNA Isolation Techniques
3 RNA Isolation Techniques
4 cDNA Synthesis
5 rDNA Library Construction
6 Library Screening
7 Nucleic Acid Hybridization
8 Expression Libraries
9 Expression Library Screening
10 DNA Sequencing Analysis
11 Secondary Structure and Functional Domain Analysis by Computers
12 Functional Analysis
13 Interaction Analysis
14 Bioinformatic Applications
Materials
Materials are not specified.
Resources
Course Assessment
Assesment MethodsPercentage (%)Assesment Methods Title
Final Exam40Final Exam
Midterm Exam20Midterm Exam
Project40Project
L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes