PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
BIO 535	NUCLEIC ACID ANALYSES	3 + 0	2nd Semester	7,5

COURSE DESCRIPTION

Course Level	Master's Degree
Course Type	Elective
Course Objective	All procedures used in molecular biology and biochemistry laboratories for the analysis of nucleic acid are given as much as detailed. The main objective of this course to give fundamental, skills to students to carry out successful experiments.
Course Content	Major topics include, Media Preparation and Bacteriological Tools, Introduction to Plasmid Biology, Minipreps & Large-Scale Preparation f, Plasmid DNA, Introduction of Plasmid DNA into Cells, Introduction to Lambda Phages, Preparation of Genomic DNA, Resolution & Recovery of DNA Fragments, Agarose Gel Electrophoresis, Capillary Electrophoresis of DNA, Southern Blotting, Hybridization Analysis of DNA Blots, Restriction Endonucleases & Restriction Mapping, Enzymes for Modifying and Radioactively Labeling Nucleic Acids, Construction of Hybrid DNA Molecules, Preparation & Analysis of RNA from Eukaryotic And Prokaryotic Cells, Production Of Genomic DNA And cDNA Libraries, Specialized Strategies For Screening Libraries, DNA Sequencing.
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	Will be able to know how to prepare plasmid
2	Will be able to know how to get genomic DNA
3	Will be able to know agarose electrophoresis
4	Will be able to know southern blotting
5	Will be able to know southern blotting
6	Will be able to know DNA and RNA sequencing
7	Will be able to know mutation analysis

COURSE'S CONTRIBUTION TO PROGRAM

	PO 01	PO 02	PO 03	PO 04	PO 05	PO 06	PO 07	PO 08	PO 09	PO 10	PO 11
LO 001		4		4			4	4	4	5
LO 002	5	5	4	5		5		5
LO 003			3	4	4
LO 004		4		4		4		4	4
LO 005		4	5	4				5		5
LO 006		4	4		3
LO 007	5		5	5
Sub Total	10	21	21	26	7	9	4	18	8	10
Contribution	1	3	3	4	1	1	1	3	1	1	0

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	3	42
Hours for off-the-classroom study (Pre-study, practice)	14	7	98
Assignments	1	5	5
Mid-terms	1	15	15
Final examination	1	35	35
Total Work Load ECTS Credit of the Course			195 7,5

COURSE DETAILS

Select Year

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester
BIO 535	NUCLEIC ACID ANALYSES	3 + 0	1	Turkish	2022-2023 Spring

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Prof. Dr. ŞEVKİ ARSLAN	sevkia@pau.edu.tr		FEN B0010	%

Goals		All procedures used in molecular biology and biochemistry laboratories for the analysis of nucleic acid are given as much as detailed. The main objective of this course to give fundamental, skills to students to carry out successful experiments.
Content		Major topics include, Media Preparation and Bacteriological Tools, Introduction to Plasmid Biology, Minipreps & Large-Scale Preparation f, Plasmid DNA, Introduction of Plasmid DNA into Cells, Introduction to Lambda Phages, Preparation of Genomic DNA, Resolution & Recovery of DNA Fragments, Agarose Gel Electrophoresis, Capillary Electrophoresis of DNA, Southern Blotting, Hybridization Analysis of DNA Blots, Restriction Endonucleases & Restriction Mapping, Enzymes for Modifying and Radioactively Labeling Nucleic Acids, Construction of Hybrid DNA Molecules, Preparation & Analysis of RNA from Eukaryotic And Prokaryotic Cells, Production Of Genomic DNA And cDNA Libraries, Specialized Strategies For Screening Libraries, DNA Sequencing.

Topics

Weeks	Topics
1	Escherichia coli, Plasmids, and Bacteriophages I
2	Escherichia coli, Plasmids, and Bacteriophages II
3	Preparation and Analysis of DNA I
4	Preparation and Analysis of DNA II
5	Enzymatic Manipulation of DNA and RNA I
6	Enzymatic Manipulation of DNA and RNA II
7	Preparation and Analysis of RNA I
8	Preparation and Analysis of RNA II
9	Construction of Recombinant DNA Libraries
10	Screening of Recombinant DNA Libraries I
11	Screening of Recombinant DNA Libraries II
12	DNA Sequencing
13	Mutagenesis of Cloned DNA
14	Introduction of DNA into Mammalian Cells

Materials

Materials are not specified.

Resources

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	50	Final Exam
Midterm Exam	50	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student