PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
BIY 214	LABORATORY ANALYSIS AND TECHNIQUES	2 + 2	4th Semester	6,5

COURSE DESCRIPTION

Course Level	Bachelor's Degree
Course Type	Compulsory
Course Objective	This course is designed for enterance of the instrumental analysis of biological samples. At the end of this course, students have good backround to work with industry and the biomedical fields
Course Content	In this course, Spectroscopy (Atomic Emission and Absorption Spectrophotometer), Differential and Density Gradient Centrifugation, Chromatography (Gel Filtration, Ion Exchange, Adsorption, Affinity Chromatography, Gas and Liquid Chromatography, Mass Spectrophotometer), Capillary Electrophoresis, Electrophoresis and Isoelectric Focusing; immunoblotting; Radiochemistry, Techniques in Describing Biological (immunological, enzymatic or receptor-based) and Electroanalytical Methods (potentiometer) Theoretical and practical aspects of techniques used, such as mentions.
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	1) give examples to oncentration units
2	2) prepare solution and buffers
3	3) solve the concentration problems
4	4) establishe a relationship between the degree of acidity or alkanitea and pH and pOH
5	5) is aware of where the centrifugation technique used.
6	6) describes the basic mechanics of the Electrophoresis
7	7) analyse the proteins by Western blot
8	8) explain the details of Spektrometric techniques.
9	9) Describes the functions of the devices used in recombinant DNA techniques.
10	10) and is aware of the relevant terms of Mammalian cell culture and devices,
11	11) Solve how all of the above techniques to do
12	12) use all of these technique uses in biological research.
13	13)prepare a draft of a research homework related with how the proteins are prufied.

COURSE'S CONTRIBUTION TO PROGRAM

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	13	3	39
Hours for off-the-classroom study (Pre-study, practice)	13	2	26
Assignments	13	2	26
Mid-terms	1	13	13
Laboratory	13	2	26
Final examination	1	26	26
Report / Project	13	1	13
Total Work Load ECTS Credit of the Course			169 6,5

COURSE DETAILS

Select Year

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester
BIY 214	LABORATORY ANALYSIS AND TECHNIQUES	2 + 2	1	Turkish	2023-2024 Spring

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Asts. Prof. Dr. REFİKA CEYDA BERAM	rberam@pau.edu.tr		FEN B0111 FEN BB107	%80

Goals		This course is designed for enterance of the instrumental analysis of biological samples. At the end of this course, students have good backround to work with industry and the biomedical fields
Content		In this course, Spectroscopy (Atomic Emission and Absorption Spectrophotometer), Differential and Density Gradient Centrifugation, Chromatography (Gel Filtration, Ion Exchange, Adsorption, Affinity Chromatography, Gas and Liquid Chromatography, Mass Spectrophotometer), Capillary Electrophoresis, Electrophoresis and Isoelectric Focusing; immunoblotting; Radiochemistry, Techniques in Describing Biological (immunological, enzymatic or receptor-based) and Electroanalytical Methods (potentiometer) Theoretical and practical aspects of techniques used, such as mentions.

Topics

Weeks	Topics
1	Gives examples to concentration units
2	Preparing solution and buffers
3	Solves the concentration problems
4	Establishing a relationship between the degree of acidity or alkanite and pH and pOH
5	Learning the centrifugation techniques used.
6	Describes the basic mechanics of the electrophoresis
7	Analyses the proteins by Western blot method
8	Explains the details of Spectrometric techniques.
9	Describes the functions of the devices used in recombinant DNA techniques.
10	Analyses the relevant terms of Mammalian cell culture and devices,
11	Solve how all of the above techniques to do.
12	Use all of these technique used in biological research.
13	Prepare a draft of a research homework related with how the proteins are prufied.
14	Prepare a draft of a research homework related with how the proteins are prufied.

Materials

Materials are not specified.

Resources

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	60	Final Exam
Midterm Exam	40	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student