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COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
BIY 306BIOCHEMISTRY LABORATORY0 + 36th Semester3

COURSE DESCRIPTION
Course Level Bachelor's Degree
Course Type Compulsory
Course Objective Perform biochemical studies using modern methods of laboratory experiments including spectrophotometry, chromatography, centrifugation, enzyme kinetics, isolation, purification and characterization proteins, nucleic acids and subcellular organelles,
Course Content Acid-Base Behavior of an amino acid pKa of a buffer to the Spectrophotometric Determination of Pigments by Spectrophotometric Analysis of Protein Carbohydrate Analysis of sugar, oils Qualitative Analysis Qualitative Analysis of Proteins in Body Fluids Isolation of microsomes Fractionation Enzyme Gel-Filtration Chromatography Detection of cellular proteins by SDS-PAGE analysis of proteins and glutathione S Ion Exchange Chromatography Characterization of Rat liver Glutathione S-Transferaz'ların-Transferaz'ların Isolation
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES
1how to determine a buffer Pka
2explains acid-base behavior of an amino acids.
3spectrophotometric analysis of proteins.
4spectrophotometric analysis of carbohydrates.
5qualitative analysis of sugars and qualitative analysis of lipids.
6detects enzymes in body fluids
7analyses isolation of microsomes.
8analysis of proteins by gel-filtration chromatography
9analyzes of proteins by ion-exchange chromatography
10analysis of proteins with SDS-PAGE
11isolation and analyzsis of glutathione S-Transferases
12Characterization of rat liver glutathione S-transferase

COURSE'S CONTRIBUTION TO PROGRAM
PO 01PO 02PO 03PO 04PO 05PO 06PO 07PO 08PO 09PO 10PO 11PO 12
LO 001            
LO 0024344 344    
LO 003            
LO 004 343        
LO 0052434        
LO 006   2 3  3   
LO 007 233        
LO 008            
LO 0093 45   4 32 
LO 0101 2         
LO 0112 31   123  
LO 012            
Sub Total12122322 649562 
Contribution112201010100

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)13339
Mid-terms11313
Final examination11313
Report / Project13113
Total Work Load

ECTS Credit of the Course






78

3
COURSE DETAILS
 Select Year   


 Course TermNoInstructors
Details 2023-2024 Spring1RAŞİT URHAN
Details 2022-2023 Spring1ŞEVKİ ARSLAN
Details 2021-2022 Spring1ŞEVKİ ARSLAN
Details 2020-2021 Spring1REFİKA CEYDA BERAM
Details 2019-2020 Spring1YEŞİM KARA
Details 2018-2019 Spring1ŞEVKİ ARSLAN
Details 2017-2018 Spring1ŞEVKİ ARSLAN
Details 2016-2017 Spring1ALİ RAMAZAN ALAN
Details 2015-2016 Spring1NAZİME MERCAN DOĞAN
Details 2014-2015 Spring1ŞEVKİ ARSLAN
Details 2013-2014 Spring1ŞEVKİ ARSLAN
Details 2013-2014 Spring2NAZİME MERCAN DOĞAN
Details 2012-2013 Spring1RAMAZAN MAMMADOV
Details 2011-2012 Spring1SERDAR DÜŞEN
Details 2010-2011 Spring1ALAATTİN ŞEN


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Course Details
Course Code Course Title L+P Hour Course Code Language Of Instruction Course Semester
BIY 306 BIOCHEMISTRY LABORATORY 0 + 3 1 Turkish 2023-2024 Spring
Course Coordinator  E-Mail  Phone Number  Course Location Attendance
Prof. Dr. RAŞİT URHAN rurhan@pau.edu.tr FEN BB107 %
Goals Perform biochemical studies using modern methods of laboratory experiments including spectrophotometry, chromatography, centrifugation, enzyme kinetics, isolation, purification and characterization proteins, nucleic acids and subcellular organelles,
Content Acid-Base Behavior of an amino acid pKa of a buffer to the Spectrophotometric Determination of Pigments by Spectrophotometric Analysis of Protein Carbohydrate Analysis of sugar, oils Qualitative Analysis Qualitative Analysis of Proteins in Body Fluids Isolation of microsomes Fractionation Enzyme Gel-Filtration Chromatography Detection of cellular proteins by SDS-PAGE analysis of proteins and glutathione S Ion Exchange Chromatography Characterization of Rat liver Glutathione S-Transferaz'ların-Transferaz'ların Isolation
Topics
WeeksTopics
1 pKa of a buffer
2 Acid-Base Behavior of an amino acid
3 Spectrophotometric Analysis of Protein
4 Spectrophotometric Determination of Pigments
5 Carbohydrate Analysis of sugar,
6 Qualitative Analysis of oils
7 Qualitative Analysis of Proteins in Body Fluids
8 exam week
9 Isolation of microsomes Fractionation
10 Enzyme Gel-Filtration
11 Chromatography Detection of cellular proteins by SDS-PAGE Transferaz'ların Isolation
12 Iolation of glutathione S transferases
13 Characterization of Rat liver Glutathione S-transferases
14 Ion Exchange Chromatography
Materials
Materials are not specified.
Resources
Course Assessment
Assesment MethodsPercentage (%)Assesment Methods Title
Final Exam40Final Exam
Midterm Exam30Midterm Exam
Quiz10Quiz
Homework20Homework
L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes