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COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
BIO 540PROTEIN ANALYSES TECHNIQUES3 + 01st Semester7,5

COURSE DESCRIPTION
Course Level Doctorate Degree
Course Type Elective
Course Objective All procedures used in molecular biology and biochemistry laboratories for the analysis of proteins are given as much as detailed. The main objective of this course to give fundamental, skills to students to carry out successful experiments in molecular biology laboratories.
Course Content Analysis of Proteins, Quantitation of Proteins, Electrophoretic Separation of Proteins, One-Dimensional SDS Gel Electrophoresis of Proteins, Two-Dimensional Gel Electrophoresis Using the ISO-DALT System, Overview of Digital Electrophoresis Analysis, Detection of Proteins, Staining Proteins in Gels & on Blot Transfer Membranes, Immunoblotting and Immunodetection, Purification of Proteins by Conventional Chromatography, Gel-Filtration Chromatography, Ion-Exchange Chromatography, Immunoaffinity Chromatography, Metal-Chelate Affinity Chromatography, Purification of Proteins by High-Performance Liquid Chromatography, Purification of Proteins by Precipitation, Immunoprecipitation, Synthesizing Proteins In vitro by Transcription and Translation, Metabolic Labeling of Aminco Acids, Isolation of Proteins for Microsequence Analysis, Capillary Electrophoresis of Proteins and Peptides, Peptide and Protein Analysis by Mass Spectrometry, Immunoassays, Enzyme-Linked Immunosorbent.
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES
1Will be able to know how to determine proteins
2Will be able to know how to carry SDS-PAGE
3Will be able to know immunochemical analyses of proteins
4Will be able to know chromatographic separations of proteins
5Will be able to know purification of proteins
6Will be able to know in vitro synthesis of proteins
7Will be able to know sequencing of proteins

COURSE'S CONTRIBUTION TO PROGRAM
Data not found.

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)14342
Hours for off-the-classroom study (Pre-study, practice)12784
Mid-terms12323
Final examination14646
Total Work Load

ECTS Credit of the Course






195

7,5
COURSE DETAILS
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L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes