Print

COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
KIM 514PROTEIN SPECIFICATION METHODS3 + 01st Semester7,5

COURSE DESCRIPTION
Course Level Master's Degree
Course Type Elective
Course Objective To provide information about understanding of methods of protein specification.
Course Content General introduction of protein synthesis, Purity determination methods, Size molecular weight and subunit analysis, Aminoacid analysis, peptide map, cofactor analysis and protein modifications analysis, crystallization methods, radioactive labelling methods, immunological anaysis (Western blot), structure anaysis
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES
1Learns the concepts of amino acid, peptide and protein.
2Learns the qualitative and quantitative determination methods of proteins.
3Understands the principles of the methods used in the isolation and purification of proteins and purity tests.
4Learns how to perform sequence analysis of proteins. Understands how to identify proteins using chromatography-mass spectroscopy techniques.

COURSE'S CONTRIBUTION TO PROGRAM
PO 01PO 02PO 03PO 04PO 05PO 06PO 07PO 08PO 09PO 10PO 11
LO 0014   3     1
LO 002    33    3
LO 0033   22    1
LO 0043   34     
Sub Total10   119    5
Contribution30003200001

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)14342
Hours for off-the-classroom study (Pre-study, practice)14684
Mid-terms12525
Laboratory5420
Final examination12424
Total Work Load

ECTS Credit of the Course






195

7,5
COURSE DETAILS
 Select Year   


 Course TermNoInstructors
Details 2018-2019 Fall1ÜMİT DİVRİKLİ
Details 2013-2014 Fall1HATİCE ARDAĞ AKDOĞAN
Details 2012-2013 Spring1HATİCE ARDAĞ AKDOĞAN
Details 2011-2012 Spring1HATİCE ARDAĞ AKDOĞAN


Print

Course Details
Course Code Course Title L+P Hour Course Code Language Of Instruction Course Semester
KIM 514 PROTEIN SPECIFICATION METHODS 3 + 0 1 Turkish 2018-2019 Fall
Course Coordinator  E-Mail  Phone Number  Course Location Attendance
Prof. Dr. ÜMİT DİVRİKLİ udivrikli@pau.edu.tr FEN A0004 %
Goals To provide information about understanding of methods of protein specification.
Content General introduction of protein synthesis, Purity determination methods, Size molecular weight and subunit analysis, Aminoacid analysis, peptide map, cofactor analysis and protein modifications analysis, crystallization methods, radioactive labelling methods, immunological anaysis (Western blot), structure anaysis
Topics
WeeksTopics
1 General introduction of protein synthesis
2 Purity determination methods
3 Purity determination methods
4 Size molecular weight and subunit analysis
5 Size molecular weight and subunit analysis
6 Aminoacid analysis
7 Peptide map
8 cofactor analysis and protein modifications analysis
9 cofactor analysis and protein modifications analysis
10 crystallization methods
11 radioactive labelling methods
12 immunological anaysis (Western blot)
13 immunological anaysis (Western blot)
14 structure anaysis
Materials
Materials are not specified.
Resources
ResourcesResources Language
D. L. Nelson, M M. Cox, ”Lehninger Principles of Biochemistry”, Worth Publishers, 2000. English
Course Assessment
Assesment MethodsPercentage (%)Assesment Methods Title
Final Exam50Final Exam
Midterm Exam50Midterm Exam
L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes