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COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
BIO 609EXPERIMENTAL ENZYMOLOGY2 + 41st Semester7,5

COURSE DESCRIPTION
Course Level Master's Degree
Course Type Elective
Course Objective
Course Content This laboratory course is concerned with molecular and BIOchemical aspects of enzymology which is the purification and structural/functional characterization of enzymes. Techniques are homogenization, to obtain cell free extract, centrifugation to separate membrane from soluble proteins, initial separations by ammonium sulfate precipitation and purifications by chromatography (anion/cation exchange, hydrophobic interaction, gel filtration, HPLC, and several concentration methods). Assessment protocols such as 1D or 2D gel electrophoresis and, western blotting combined with colorimetric protein concentration determinations, functional characterization with activity assays using UV/VIS and various spectroscopies. For further characterization MALDI-TOF technique will be discussed in theory.
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES

COURSE'S CONTRIBUTION TO PROGRAM
Data not found.

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)14342
Hours for off-the-classroom study (Pre-study, practice)14798
Assignments155
Mid-terms11515
Final examination13535
Total Work Load

ECTS Credit of the Course






195

7,5
COURSE DETAILS
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L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes