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COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
BIO 533MOLECULAR BIOLOGY TECHNIQES3 + 02nd Semester7,5

COURSE DESCRIPTION
Course Level Master's Degree
Course Type Elective
Course Objective To explain the techniques used in molecular biology.
Course Content E. coli Genetics, Preparation of Plasmids, Phages, Vectors, İzolation and Analysis of DNA and RNA, Preparation of Genomic DNA, PCR Analysis, Agaroze Gel Electrophoresis, Southern Blot Analysis, Northern Blot Analysis, Enzyme Treatments of DNA and RNA, Restriction Mapping, Radyoaktive Tagging, Preparation of Genomic and CDNA Libraries, Screening of Genomic and CDNA Libraries, Gene Bank. .
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES
1Explain E. coli genetics.
2Describes preparation of plasmids, phages and vectors.
3Explain the methods of isolation and analysis of DNA and RNA.
4Explain enzyme treatments of DNA and RNA.

COURSE'S CONTRIBUTION TO PROGRAM
PO 01PO 02PO 03PO 04PO 05PO 06PO 07PO 08PO 09PO 10PO 11
LO 0014 34 3553  
LO 002 454    34 
LO 0033543       
LO 004  45   43  
Sub Total791616 35994 
Contribution22440112210

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)14342
Hours for off-the-classroom study (Pre-study, practice)14798
Assignments155
Mid-terms11515
Final examination13535
Total Work Load

ECTS Credit of the Course






195

7,5
COURSE DETAILS
 Select Year   


 Course TermNoInstructors
Details 2020-2021 Spring1FEVZİYE ÇELEBİ TOPRAK
Details 2014-2015 Spring1FEVZİYE ÇELEBİ TOPRAK
Details 2012-2013 Spring1FEVZİYE ÇELEBİ TOPRAK


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Course Details
Course Code Course Title L+P Hour Course Code Language Of Instruction Course Semester
BIO 533 MOLECULAR BIOLOGY TECHNIQES 3 + 0 1 Turkish 2020-2021 Spring
Course Coordinator  E-Mail  Phone Number  Course Location Attendance
Prof. Dr. FEVZİYE ÇELEBİ TOPRAK fctoprak@pau.edu.tr FEN B0310 %80
Goals To explain the techniques used in molecular biology.
Content E. coli Genetics, Preparation of Plasmids, Phages, Vectors, İzolation and Analysis of DNA and RNA, Preparation of Genomic DNA, PCR Analysis, Agaroze Gel Electrophoresis, Southern Blot Analysis, Northern Blot Analysis, Enzyme Treatments of DNA and RNA, Restriction Mapping, Radyoaktive Tagging, Preparation of Genomic and CDNA Libraries, Screening of Genomic and CDNA Libraries, Gene Bank. .
Topics
WeeksTopics
1 Introduction to Molecular Techniques
2 Isolation Techniques of DNA and RNA
3 Analysis of DNA and RNA I
4 Analysis of DNA and RNA II
5 Usage of Bacteria, Plasmid and phage I
6 Usage of Bacteria, Plasmid and phage II
7 Usage of Bacteria, Plasmid and phage III
8
9
10
11
12
13
14
Materials
Materials are not specified.
Resources
Course Assessment
Assesment MethodsPercentage (%)Assesment Methods Title
Final Exam50Final Exam
Midterm Exam50Midterm Exam
L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes